B.Sc. (Hons) Biochemistry,  1990,  University of Southampton, United Kingdom

Ph.D Biochemistry, 1994,  University of Southampton, United Kingdom

Plant functional genomics and molecular biology

Molecular biology of fruit ripening

Functional genomics of seaweed

Penyelidikan IRPA

0902020107  Cloning and recombinant expression of papaya (carica papaya L) a-galactosidase

09-02-02-002-BTK/TD/003  Microbial genomics for gene and general product discovery

09-02-02-0040-EA136 Identification of genes involved in pathogenicity from the plant pathogen Colletotrichum gloeosporioides

01-02-02-0018-EA194  Chilling injury alleviation for storage life extensin in papaya : The biochemical and molecular bases of texture changes and ethylene action

01-02-02-0017-EA176  Role of subtilisin in ripening of selected tropical fruits

06-02-02-007-BTK/ER/32/02  Identification and analysis of genes/underlying allergic diseases using cDNA microarrays

1.               Sumathi Balasubramaniam, Heng Chin Lee, Hamid Lazan, Roohaida Othman dan Zainon Mohd Ali (2005) Purification and properties of a beta-galactosidase from carambola fruit with significant activity towards cell wall polysaccharides. Phytochemistry 66: 153-163.

2.               Roohaida Othman, Diana Mohd Nor, Srinda Swaminy Nathan, Chan Poh Chu, Rahmah Mohamed dan Wan Kiew Lian (2004) A large scale gene expression analysis in red alga Eucheuma denticulatum. Pros. Simp. Biol. Keb. 7: 321-324.

3.               Siti Norsuha Misman, Zulkeflie Zamrod, Farah Diba Abu Bakar, Roohaida Othman, Abdul Munir Abdul Murad dan Nor Muhammad Mahadi (2004) Cloning and characterisation of a cutinase transcription factor gene from Colletotrichum gloeosporiodes. Pros. Simp. Biol. Keb. 7: 417-420.

4.               Shen Wai Sen, Hasidah Mohd Sidek, Md Sharom Md Yusof dan Roohaida Othman, 2004, Kesan ekstrak kasar rumpai laut merah Eucheuma denticulatum ke atas aktiviti anti-inflamasi. Pros. Sem. IRPA RMK 8 Kategori PR/SR/BTK 2004, 2: 149-152.

5.               Nor Azfa Johar, Hasidah Mohd Sidek dan Roohaida Othman, 2004, Kesan ekstrak Glacilaria changii ke atas pengekspresan gen protein tirosin kinase dan fosfatase pada sel kanser serviks (HeLa) dan ovari (CaOV-3). Pros. Sem. IRPA RMK 8 Kategori PR/SR/BTK 2004, 2: 153-156.

6.               Roohaida Othman, Diana Mohd Nor, Srinda Swaminy Nathan, Chan Poh Chu, Rahmah Mohamed dan Wan Kiew Lian (2004) Analisis pengekspresan gen dalam rumpai laut merah Eucheuma denticulatum. Pros. Sem. IRPA RMK 8 Kategori PR/SR/BTK, 2: 157-160.

7.               Roslan Harun, Jeenath Banu Jamaludin, Roohaida Othman dan Mohd Saifulaman Mohd Said, 2004, Identifikasi gen yang terlibat dalam perkembangan CD4+VE T limfosit dalam asma dan atopi dengan menggunakan mikroatur. Pros. Sem. IRPA RMK 8 Kategori PR/SR/BTK 2004, 2: 161-164.

8.               Tan Bee Aik, Zainon Mohd Ali, Roohaida Othman dan Hamid Lazan (2004) Expression of beta-galactosidase gene in E.coli system, Program book of 29th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology.

9.               Kasazlinda Jamal, Chong Jin Yee, Roohaida Othman, Zainon Mohd Ali dan Hamid Lazan (2004) Isolation and characterisation of papaya SAM synthase cDNA clone, Programme book of 29th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology.

10.           Chong Huei Li, Roohaida Othman, Zainon Mohd Ali dan Hamid Lazan (2004) Isolation of papaya beta-galactosidase cDNA clone during ripening, Programme book of 29th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology.

11.           Nuraziyan Azimi dan Roohaida Othman (2004) Cloning of papaya subtilisin cDNA, Programme book of 29th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology.

12.           Chong Huei Li dan Roohaida Othman (2004) Isolation of papaya peptidase cDNA clone during ripening, Programme book of 15th Malaysian Society of Plant Physiology Conference.

13.           Roohaida Othman, Diana Mohd Nor, Srinda Nathan, Chan Poh Chu, Rahmah Mohamed dan Wan Kiew Lian (2004) Analysis of expressed sequence tags from Eucheuma denticulatum (rhodophyta), Programme book of 6th Asia Pacific Marine Biotechnology Conference.

14.           Akzam Saidin, Rahmah Mohamed dan Roohaida Othman (2004) Cloning of sulfotransferase gene from Eucheuma denticulatum. Abstract book of International Conference on Bioinformatics 2004.

15.           Salmijah Surif, Faridah Shah, Wan Kiew Lian dan Roohaida Othman (2004) Search for biomarkers for heavy metal exposure in the aquatic environment using blood cockle Anadara granosa as a model organism. Malaysian J. Biochem. Mol. Biol. 9: 51.

16.           Kasazlinda Jamal, Roohaida Othman, Soh Chin Pin, Zainon Mohd Ali dan Hamid Lazan, (2004) Cloning of papaya alpha-galactosidase cDNA, Malaysian J. Biochem. Mol. Biol. 9: 89.

17.           Chin Chui Yoke, Roohaida Othman dan Sheila Nathan (2004) Cloning, expression and purification of Burkholderia pseudomallei serine metalloprotease, SMBPF4 from local isolate, Abstract book 4th World Melioidosis Congress 2004, Singapore, Bahasa Inggeris, Salinan Keras (Kertas),.

18.           Roohaida Othman, Tan Wei Fui, Zainon Mohd Ali and Hamid Lazan (2003) A fruit specific and ripening related endopolygalacturonase gene from papaya. Trans. Malaysian Soc. Plant Physiol. 12:26-30.

19.              Norhatiah Md. Lias, Farah Diba Abu Bakar, Roohaida Othman, Zulkeflie Zamrod, Sheila Nathan, Abdul Munir Abdul Murad dan Nor Muhammad Mahadi (2003) Cloning and expression of the Glomerella cingulata cutinase recombinant gene in the yeast, Pichia pastoris, Proc. Symp. Appl. Biol. 7: 241-244.

20.              Fauziah Hanim Shaarani, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Sheila Nathan, Raja Noor Zaliha Raja Abd Rahman, Zulkeflie Zamrod, Roohaida Othman dan Nor Muhammad Mahadi (2003) Conformation and binding interaction studies on Glomerella cingulata cutinase. Proc. Symp. Appl. Biol. 7: 262-264.

21.              Farah Diba Abu Bakar, Zulkeflie Zamrod, Abdul Munir Abdul Murad, Roohaida Othman, Rohaiza Ahmad Redzuan, Siti Norsuha Misman, Mohd Syahid Ihsamuddin dan Nor Muhammad Mahadi (2003) Identification of genes involved in pathogenicity from the plant pathogen, Colletotrichum gloeosporioides. Pros. Sem. IRPA RMK-8 Kategori EAR, 2: 279-282.

22.              Hamid Lazan, Nor Mayati Che Husin, Razean Haireen Mohd Razali, Soh Chin Pin, Roohaida Othman dan Zainon Mohd Ali (2003) Ethylene biosynthesis and perception in relation to expression of softening enzymes in developing and ripening papaya fruits, Biology and Biotechnology of the Plant Hormone Ethylene III: 64-65.

23.              Farah Diba Abu Bakar, Roohaida Othman, Syazrah MA, Norhatiah Md Lias, Zulkeflie Zamrod, Abdul Munir Abdul Murad, Sheila Nathan and Nor Muhammad Mahadi (2003) Expression of a cutinase G. cingulata recombinant gene in E.coli.  Malaysian J. Biochem. Mol. Biol. 8: 72.

24.              Norazah MN, Rahimah Abd Rahim, Suwaibah S, Siti Norsuha Misman, Farah Diba Abu Bakar, Zulkeflie Zamrod, Roohaida Othman, Abdul Munir Abdul Murad dan Nor Muhammad Mahadi (2003) Analysis of a hygromycin resistant cassette and its use in gene disruption and promoter activity studies in fungi.  Malaysian J. Biochem. Mol. Biol. 8: 78.

25.              Lawrence Sie, Roohaida Othman, Zainon Mohd Ali dan Hamid Lazan (2003) Differential gene expression of heat treated papaya. Malaysian J. Biochem. Mol. Biol. 8: 91.

26.              Chan Peng Yeow, Roohaida Othman, Zainon Mohd Ali dan Hamid Lazan (2003) Isolation of Papaya ripening related cDNA involved in isoprenoid biosynthesis. Malaysian J. Biochem. Mol. Biol. 8: 92.

27.              Wong Kok Kee, Roohaida Othman and Salmijah Surif (2003) Isolation of copper induced genes in bolood cockles.  Malaysian J. Biochem. Mol. Biol. 8: 98.

28.              Tan Wei Fue, Roohaida Othman, Zainon Mohd Ali dan Hamid Lazan (2003) The role of V-ATPase in tissue softening during papaya ripening.  Malaysian J. Biochem. Mol. Biol. 8: 99.

29.              Chan Pek Lan, Siti Nor Akmar Abdullah and Roohaida Othman (2003) Isolation and characterization of a full-length leaf specific gene from the oil palm.  Malaysian J. Biochem. Mol. Biol. 8: 106.

30.              Nor Azfa Johar, Hasidah Mohd Sidek dan Roohaida Othman (2003) Tyrosine kinase and phosphatase gene expression in cervical and ovarian cancer cell lines: effect of Gracilaria changii crude extract, Programme book 2nd International Conference on Bioinformatics, 8 - 10 September 2003, Penang.

31.              Nor Azfa Johari, Hasidah Mohd Sidek dan Roohaida Othman (2003) Expression of tyrosine kinase and phosphatase genes in CaOV-3 cells and the effect of G.changii treatment, Programme book UMS Biotechnology Symposium II. Signal Transduction: Mechanisms & Applications, 3 - 5 Disember 2003, Univ Malaysia Sabah, K. Kinabalu.

32.              Nor Azfa Johari, Hasidah Mohd Sidek dan Roohaida Othman (2003) Tyrosine kinase and phosphatases as drug targets in G.changii treated CaOV-3 cells, Programme book 14th National Biotechnology Seminar, 11 -13 Disember 2003, Hotel Resort Mutiara, Penang.

33.              Roohaida Othman, Lawrence Sie Eng Kean, Zainon Mohd Ali dan Hamid Lazan (2003) Isolation and characterisation of heat induced genes in papaya, Book of Abstracts 7th International Congress of Plant Molecular Biology, 23 - 28 June 2003, Barcelona, Spain.

34.           Chan Mooi Kwai, Roohaida Othman, Zubir Daud. And Salmijah Surif (2002) Induction of putative metalothionein gene in the blood cockles (Anadara granosa) exposed to cadmium. Comparative Biochemistry and Physiology Part C 131: 123-132.

35.           Hamid Lazan, Nor Mayati Che Husin, Soh Chin Pin, Roohaida Othman, Razean Haireen Mohd. Razali and Zainon Mohd. Ali (2002) Ethylene biosynthesis and perception in relation to expression of softening enzymes in developing and ripening papaya fruits. Biology And Biotechnology Of The Plant Homone Ethylene III: 64-65.

36.              Roohaida Othman, Lawrence Sie Eng Kean, Zainon Mohd Ali dan Hamid Lazan (2002), Expression of glutahione-s-transferase in papaya after heat shock and cold storage, Trans. Malaysian Soc. Plant Physiol. 11: 144 - 146.

37.           Soh, C. P., Ali, Z. M., Tham, C. H., Tay, W. S., Othman, R. and Lazan, H. (2001) Cell wall ultrastructure and disassembly during ripening of papaya fruit. Trans. Malaysian Soc. Plant Physiol. 10:33-36.

38.           Soh, C. P., Ali, Z. M., Othman, R. and Lazan, H. (2001) Evidence of interactions among softening enzymes in affecting cell wall modifications during fruit ripening. Trans. Malaysian Soc. Plant Physiol. 10:37-40.

39.           Ali, Z. M., Soh, C. P., Razali, R. H. M., Husin, N. M. C., Ng, S. Y., Othman, R. and Lazan, H. (2001) Expression of softening genes and proteins in ripening papaya fruit. Trans. Malaysian Soc. Plant Physiol. 10:41-43.

40.           Othman, R., Jamal, K., Ali, Z. M., Soh, C. P. and Lazan, H. (2001) PCR amplification and cloning of papaya a-galactosidase. Trans. Malaysian Soc. Plant Physiol. 10:44-46.

41.           Lau, H. Y., Zainal, Z., Ismail, I., Sidik, N. M. and Othman, R. (2000) Differential screening of chilli cDNA library. Trans. Malaysian Soc. Plant Physiol. 9: 125-128.

42.           Sajari, R., Zainal, Z., Sidik, N. M., Ismail, I., and Othman, R. (2000) Isolation of a set of ripening-related cDNA clones from chilli by differential screening. Trans. Malaysian Soc. Plant Physiol. 9: 147-150.

43.           Othman, R., Soo, K. K. Ali, Z. M. and Lazan, H. (2000) Isolation of papaya subtilisin cDNA clone. Trans. Malaysian Soc. Plant Physiol. 9: 151-154.

44.           Othman, R., Soo, K. K. Ali, Z. M. dan Lazan, H. (2000) Pemencilan klon cDNA bagi subtilisin buah betik. Seminar IRPA-UKM 2: 37-41.

45.           Lazan, H., Husin, N. M. C., Ali, Z. M., Razali, R. H. M. dan Othman, R. (2000) Pengekspresan terdiferensiasi gen terlibat dalam biosintesis dan persepsi etilena serta pelembutan semasa perkembangan buah betik. Seminar IRPA-UKM 2: 42-47.

46.           Chan, M. K., Othman, R., Daud, Z. dan Salmijah, S. (2000) Induksi pengekspresan gen metalotionin oleh kadmium dalam kerang, Anadara granosa. Seminar IRPA-UKM 2: 370-374.

47.           Chan, M. K., Othman, R., Daud, Z. dan Salmijah, S. (2000) Induction of metallothionein gene in blood cockle (Anadara granosa) exposed to cadmium. Proc. Nat. Biotech. Sem. 12: 136-139.

48.           Chan, P. L., Abdullah, S. N. A. and Othman, R. (2000) Isolation of leaf-specific gene(s) from oil palm leaf cDNA library. Proc. Nat. Biotech. Sem. 12: 459-460.

49.           Zainon Mohd. Ali, Ng. Chin Hong, Chua Sok Tuang, Roohaida Othman and Hamid Lazan, 2000: Heat treatment alleviates chilling injury symptoms, retards texture changes and maintains the capacity to produce ethylene in papaya fruits. Proc. Int. Institute of Refrigeration Conf. (Murcia, Spain) 519-524.

50.           Soh Chin Pin, Zainon Mohd. Ali, Roohaida Othman and Hamid Lazan, 2000: Role of a-galactosidase II in softening of papaya fruits. Abstract of Int. Cong. Biochem. Mol. Biol. (Birmingham, UK) 18:102.

51.           Sharon F. Baker, Roohaida Othman and David C. Wilton, 1998: Tryptophan-containing mutant of human (group II) phospholipase A2 has a drammatically increased ability to hydrolyze phosphatidylcholine vesicles and cell membranes. Biochemistry 37, 13203-13211.

52.           Adrian R. Kinkaid, Roohaida Othman, Josephine Voyser and David C. Wilton 1998: Phospholipase D and phosphatidic acid enhance the hydrolysis of phospholipids in vesicles and in cell membranes by human secreted phospholipase A2. Biochim. Biophys. Acta 1390 (2), 173-185.

53.           Zainon Mohd. Ali, Ng, S. Y., Roohaida Othman, Goh, L. Y. and Hamid Lazan, 1998: Isolation, characterisation and significance of papaya b-galactanases to cell wall modification and fruit softening during ripening. Physiol. Planta. 104, 105-115.

54.           Choo Tze Siang, Roohaida Othman, Zainon Mohd. Ali, Zamri Zainal, and Hamid Lazan, 1998: Isolation and characterisation of a papaya (Carica papaya cv. Eksotika) b-galactosidase cDNA (Accession no :AF064786). Plant Physiol.  118, 1102.

55.           Ali, Z. M., Chin, L. H., Othman, R. and Lazan, H. (1999). Carambola and papaya fruits b-galactosidases differentially modify the cell wall of unripe carambola. Proc. IRPA-UKM Sem 2: 47-51.

56.           Othman, R., Choo, T. S., Ali, Z. M., Zainal, Z. and Lazan, H. (1999). Sequence characterisation of papaya b-galactosidase clone using computer software. Proc. IRPA-UKM Sem 2: 53-56.