• Qualifications :

    BSc.(Hon)Chem. Eng.(Leeds), PhD(QEC, London)




  • Specialisation :

    Fermentation technology, Industrial Biotechnology, Design of Waste Water Treatment Plant




  • Areas of Research :

    1. Design of biological systems (single and multistage) for the production of 

       primary and secondary metabolites.

    2. Evaluation (qualititive and quantitative) of fermentation process.

    3. Development of high density biomass production systems.

    4. Production of cellulase, CGTase, Itaconic acid, bioinsecticides, GLA,

        biotransformation of natural rubber


  • Current Projects and Grants :
      1        Status      :  Member

    Title         : Continuous Hydrogenation of Palm Oil Using Supported Catalysts

                Duration   : 1986 - 1990

                Funding    : UKM. RM 55,000

                Member   : Professor Dr. Che Husna Azhari (Project Leader)

                Outcome  : A Postgraduate student

                                  A Postgraduate Thesis

                                  Two papers in journal and several papers in proceeding

                                  A Final Report

    2          Status       : Member

                Title          : Hydrodynamic Studies In Circulating Fluidised Bed Reactor

                Duration    : 1994

                Funding     :  Suruhanjaya Sains EC-Asean RM 450,000

                Members  : Prof. Pierre Guigon      

                                  Prof. Khalil Shakourzadeh

                                  Professor Dr. Che Husna Azhari (Project Leader)

                                  Assoc. Prof. Dr. Siti Masrinda Tasirin

                Outcome  : A pilot plant -Circulating Fluidised Bed Reactor

                                  A proceeding at the National Level                     

    2                    Status      : Member

                Title         : Properties of Particle in Solid Flow

                Duration   : 1997- 1999

                Funding    : UKM RM 10,000

                Members  : Professor Dr. Che Husna Azhari (Project Leader)

                                  Assoc. Prof. Dr. Siti Masrinda Tasirin

    3                    Status       : Project Leader

                Title          : Biotransformation of Natural Rubber in Packed Bioreactor


                Duration    : 1997- 1999

                Funding     : IRPA 01-04-0040 RM 70,000

                Members   : Dr. A. Ikram (RRI) (project leader)

                                   Dr. Zainal Abidin (RRI)

                                  Professor Dr. Che Husna Azhari

    5          Status         : Member

                Title            : The Malays Technology

                Members     : Prof. Muhammad Haji Salleh SN dan Pengarah ATMA (project leader)

                                    Prof. Dr.Wan Ramli Wan Daud

                                    Prof. Dr. Che Husna Azhari

                                    Assoc. Professor Dr. Wan Mohtar Wan Yusoff

                                    Professor Dr. Kamaruddin Yusoff

                                    Professor Dr. Hassan Basri

                                    Assoc. Professor Dr. Yusoff Ali

                                    Assoc. Prof.Dr. Shahidan Radiman


    6          Status          : Project Leader

    Title            : Preliminary Evaluation of Production of Bioinsecticides from local waste materials

                Duration       : 1987 -1991

                Funding        : RM 30,000 IFS E/1068 SWEDEN

                Member       : Prof Mohd Sanusi Jangi      

                Outcome      : Several undergraduate theses

                                    Articles in proceeding                

    7          Status           : Project Leader

                Title             : Utilization of Cells and Enzyme Production to change cellulosic        


                Duration       : 1988-1993

                Funding        : IRPA 4-07-03-008

                Members      : Prof. Dr.Abdul Jalil Abdul Kader  (Project Leader)     

                                      Prof. Dr.Othman Omar

                                      Assoc. Prof. Dr. Fauzi Daud

                Outcome       : A postgraduate student

                                      Several undergraduate theses

                                      Packed Bioreactor system for fermentation  processes

                                      Articles in proceeding/journals

    8          Status            : Project Leader

                Title               : Continuous production of bioinsecticides

                Duration         : 1988 - 1993

                Funding          : IRPA 4-07-03-003

                Members        : Prof. Dr.Nor Muhammad Mahadi (project leader)

                                        Prof. Dr.Mohd Sanusi Jangi

               Outcome          : Several undergraduate theses

                                        A bioreactor system set–up for continuous production of  

                                        bioinsecticides  .

                                        Articles in proceeding/journal        

    9         Status               : Project Leader

               Title                  : Production of Itaconic Acid for Biopolymers

              Duration            : 1991 - 1995

              Funding             : MPKSN RM 25,000 BP2 9370088

              Member            : Professor  Dr. Che Husna Azhari     

              Outcome           : A Postgraduate student

                                         Several undergraduate theses

                                        Articles in proceeding

    10        Status              : Project Leader

    Title                : The production of cyclodextrin glucosyl transferace (CGT ase) by bacteria.

    Duration          : started Mac 2000

                Funding           : RM 72,000.00 KSTAS : IRPA 09-02-02-0118

                Member          : Dr. Aidil Abd. Hamid  UKM

                                        Dr. Rosli Md. Illias       UTM

                                        Dr. Noor Aini Abd. Rashid UTM


    11        Status              : project leader

                Title                 : Biotransformation of natural rubber by actinomicete #4

                Funding            : KSTAS : IRPA 01-04-04-0040

                Fund                : RM 70,000.00

                Members         : Dr. A. Ikram    LGM

                                         Prof Dr. Che Husna Azhari   UKM

    12        Status              : programme leader

                Title                 : Production of Microbial Polyunsaturated Fatty Acid

               Funding            : KSTAS : IRPA 09-02-02-001 (BTK/TD/001) Top-down RM8

                Fund                : RM 880,000 untuk 3 tahun (2001-2004)

                Members         : Dr. Aidil Abd. Hamid  UKM            secretariat

                                         Dr. Rosli Md. Illias      UTM            sub-project head

                                         Dr. Hirzun                  UPM            sub-project head

                                         Dr. Subari                   MARDI        sub-project head

                                         Assoc. Prof. Dr. Mohd Khan Ayub UKM   sub-project head


  • Selected Publications :


    1         Ismail B. Ahmad, Zubaidah A. Hassan Goh Toh Seng, Shanmugam K. Murthy,Siti Romlah Yahya, Nurina Anuar,  Wan Mohtar W. Y. & Laily B. Din (1989).  Biologi Chaetomium sp dan Kesan Perencatan Terhadap Pertumbuhan Beberapa Bakteria.  Sains Malaysiana 18(3): 55-64.


    2         Wan Mohtar Wan Yusoff & Ravindran Thayan (1990).  Cellulose degradation in a packed reactor system using mixed culture fermentations.  Journal of the Singapore National Academy of Science, Vol. 18 & 19, 13-16


    3         Wan Mohtar Wan Yusoff. &  Ravindran (1991).  Synergism of  b-glucosidase and cellulose in mixed culture fermentations.  Malays.  Appl. Biol. Vol. 20(2):215-222.


    4         Jalil Kader, Zainal Abdin Mohd Yusoff, Othman  Omar, Wan Mohtar  Wan Yusoff & Fauzi Daud (1992).  Pemencilan mikroorganisma selulolisis daripada enapcemar kelapa sawit.  Sains Malaysiana 21(5), 75-85.


    5         Wan Mohtar Wan Yusoff., Abd. Jalil Abd. Kader, Sasidharan Velayutham, Othman Omar (1992).  Effects of temperature on continuous cellulolysis by Aspergillus terreus SUK-1 in a packed reactor system.  Jurnal Sains Malaysiana (Sains Hayat) 21(3), 41-50.


    6         Che Husna Azhari, Sufian Ali & Wan Mohtar Wan Yusoff (1992).  Penghidrogenan selanjar minyak olein sawit dengan mangkin platinum tersokong.  Jurnal kejuruteraan 4:61-70.


    7         Wan Mohtar Wan Yusoff. & M.J. Bazin (1994).  Simultaneous production of cellulase and reducing sugar in a multistage packed reactor system.  ASEAN J. Sci. Technol. Develop. 11(2):107-116.


    8         Wan Mohtar Wan Yusoff., Che Husna Azhari & D. Lonchamp. (1994). Separation  by selective adsorption : Preparative Liquid Chromatography.  Journal of Industrial Technology, 4(1):79-86.


    9         Wan Mohtar Wan Yusoff., K.M. Shanmugam, & I.B. Ahmad (1995).  Cultivation of Chaetomium trilaterale Chivers for antibiotic production in submerged culture.  Malays.  Appl. Biol. 24(2):95-100.


    10     Wan Mohtar Wan Yusoff. & S. Suresh (1996).  The effect of aeration rate on itaconic acid production by entrapped Aspergillus Terreus SUK-1 in a packed bioreactor system.  Malays. Appl. Biol. 25(1):75-80.


    11     Wan Mohtar Wan Yusoff., Abd. Jalil Abd. Kader & Othman Omar (1996). Effect of reactor operation, substrates  and medium flowrate in the continuous production of cellulase enzyme in a packed reactor.  Malays.  Appl. Biol. 25(2): 81-88.


    12     Wan Mohtar Wan Yusoff, Othman O. , J.Kader, Suresh.S dan Shree Mano.J 1997. Effects of sodium chloride on cellulase production by Aspergillus terreus SUK – 1 Malays. Appl. Biol 26(1): 1-5



    13     Kader. Jalil, Getha Krishnasamy, Wan Mohtar Wan Yusoff and Othman Omar.(1999).

    Hydrolysis of empty fruit bunch of oil palm using cellulolytic enzymes from Aspergillus terreus IMI 28243.

    J. Microbiol. Biotechnol. 9 (4), 514-517.


    14     Che Husna Azhari , Siti Masrinda Tasirin, Mulfi Hazwi & Wan Mohtar Wan Yusoff (2000)

    Studies on axial solid flow structures in CFB reactors.

    Jurutera,  IEM, MALAYSIA No. 10 Bil. 2000 : 49-56


    15     Wan Mohtar Wan Yusoff , Muhannad Illayan Massadeh & Jalil Kader. (2000)

    Solid Substrate and submerged culture fermentation of sugar cane bagasse for the production of cellulase and reducing sugar by a local isolate, Aspergillus terreus IMI 28243.

    J. Microbiol. Biotechnol. 10 (5), 770-775


    16     Wan Mohtar Wan Yusoff, Muhannad I. Massadeh, Othman Omar and Jalil Kader. (2000)  Sugar cane bagasse degradation by mixed culture of T. reesei and A. terreus in solid substrate fermentation. Pakistan Journal of Biological Sciences 3 (10) : 1758-1761.


    17     Wan Mohtar M.Y, Ho Y.H and Pang W.K. (2000). The effect of cellulose in the production of itaconic acid by Aspergillus terreus IMI 28243. Malays. Appl. Biol,29 (1&2): 149-151


    18     Wan Mohtar Wan Yusoff, Pang Wey Kit , Osman Hassan & Suresh Suppiah. (2001) Influence of Cobalt, Nickel and Zinc on itaconic acid production by a local isolate Aspergillus terreus IMI 28243. Pakistan Journal of Biological Sciences 4(1) : 107-109


    19     Siti Maslinda Tasirin, Wan Mohtar W. Y, Mulfi Hazwi Mohd Dien & Che Husna Azhari (2001) Influence of Particle Size on the axial flow profile in a rectangular CFB riser. 6th World Congress of Chemical Engineering. Melbourne2001. P3-205. 1-9 Inst of Chem. Eng. Aust.


    20     A. Hamid, W.M.W. Yusoff, R.M. Illias & K. Nadarajah (2001).  Pengasingan strain baru kulat dari tanah di Malaysia untuk penghasilan asid  ?– linolenik (GLA).  Jurnal Teknologi, 34 (C) Jun 2001 : 1-8


    21     M.I. Massadeh, W.Mohtar W. Yusoff, O. Omar and J. Kader (2001). Synergism of  cellulase enzymes in mixed culture solid substrate fermentation.  Biotechnology Letters .  23: 1771-1774


    22   Wan Mohtar Wan Yusoff, Mohd Mazmira Mohd Masri, Bhavani, U. and

    Rusni Maruan (2001).  Growth and Sporulation of Bacillus thuringiensis

    subsp. aizawai SN2 in a two-stage continuous culture.  Journal of Industrial

    Technology vol. 10 No.2 ( in press)


    23    W.Y.Wan Mohtar, W.G.Loh, and H. Osman (2001). Effect of NaCl in agar medium, growth medium and production medium in the production of itaconic acid by Aspergillus terreus IMI 282743. Malays. Appl. Biol. (June & December 2001) 30(1&2) : 99-103


    24    Che Husna Azhari, Aulia Saad, Wan Mohtar Wan Yusoff and A. Ikram (2002). Biodegradation of natural rubber by Actinomycete No. 4 in a continuous culture system. Pakistan journal of Biological Sciences 5(3): 329-331.



    25    Rosli Md. Illias, Tien Siew Fen, Nor Aini Abdul Rashid, Wan Mohtar Wan Yusoff, Aidil Abd. Hamid, Osman Hassan & Kamaruzzaman Kamaruddin (2002). Cyclodextrin Glucanotransferase producing alkalophilic Bacillus species.G1: It’s cultural conditions and partial characterisation of the enzyme. Pakistan journal of Biological Sciences 5(6): 688-692.


    26  Mohd Sahaid Kalil, Pang Wey Kit, Wan Mohtar Wan Yusoff, Yoshino Sadazo and Rakmi Abdul Rahman (2003). Direct Fermentation of Palm Oil Mill Effluent to Acetone-botanol-ethanol by solvent producing Clostridia. Pakistan Journal of Biological Sciences 6 (14): 1273-1275. Asian Network for Scientific Information


    27  Wan Mohtar Wan Yusoff, Hanan Moawia Ibrahim, Aidil Abdul Hamid, Rosli Md. Illias, Osman Hassan and Othman Omar (2002). A potential new isolate for b-Cyclodextrin Glucanotransferase production. Malaysian Journal of Biochemistry and Molecular Biology. Vol 7: 13-17


    28  Mohd. Sahaid Kalil, Muhammad Zaki, Wan Mohtar Wan Yusoff & Mohammad Ramlan Mohd Salleh (2003). Sebatian metoksiaromatik sebagai substrat pertumbuhan dan pengaruh enzim demetilase dalam Acetobacterium Woodii DSM1030. Jurnal Teknologi no.38 (C), Jun 2003: 61-68


    29  Wan Mohtar,W.Y. and Mohd Mazmira, M.M. (2003). Medium formulation for improvement of growth and sporulation of Bacillus thuringiensis Subsp Kurstaki using a two-stage continuous culture. Malays. Appl. Biol.(jun 2003) 32(1): 61-67


    30  Rosli Md. Illias, Tien Siew Fen, Roshanida A Rahman, Noor Aini Abdul Rashid, Wan Mohtar Wan Yusoff, Aidil Abd. Hamid, Osman Hassan & Kamaruzzaman Kamaruddin (2003). Application of factorial design to study the effects of temperature, initial pH and agitation on the production of Cyclodextrin Glucanotransferase from alkalophilic Bacillus sp G1. Science Asia 29:135-140


    31  Hanan Moawia Ibrahim, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Rosli Md. Illias and Othman Omar (2003). Optimisation of the process conditions for Cyclodextrin Glucanotransferase production using response surface methodology. Asian J. of Microbial. Biotech. Env. Sc. 3: 297-300


    32  Wan Mohtar Wan Yusoff, Muhannad I Massadeh, Jalil Kader, Fauzi and Othman Omar (2003). Comparison of cellulolytic enzymes production by single-stage solid fermentation (SSF) and submerged culture fermentation(SCF) and the combination of SSF and SCF fermentation systems. Jurnal Biosains Vol. 14 No. 2, 7-17


    33  Pang Wey Kit, Mohd Sahaid Kalil, Osman Hassan & Wan Mohtar Wan Yusoff (2004). Penghasilan Aseton-Butanol-Etanol dan gas hydrogen daripada efluen kilang kelapa sawit secara fermentasi anaerobic dengan Clostridium acetobutylicum NCIMB 13357. Sains Malaysiana 33(1): 73-81



    34  Hanan Moawia Ibrahim, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Rosli Md. Illias, Osman Hassan, Othman Omar (2005). Optimization of medium for the production of b-cyclodextrin glucanotransferase using central composite design (CCD). Process Biochemistry 40 (2005) 753-758.


    35 Aidil, A.H., Rosli, M.I. and Wan Mohtar, W. Y. (2005). The effect of carbon and nitrogen ratios and temperature on lipid and gamma-linolenic acid (GLA) yield in four fungal isolates. Malaysian Applied Boilogy 34(1): 15-19


    36  Muhammad Zaki, Che Husna Azhari, Mohd Sahaid Kalil and Wan Mohtar Wan Yusoff (2005). Production of Novel Material through Biotransformation of Natural Rubber. Journal of Applied Technology (jurnal teknologi terpakai). Vol.2 No.2 (91-98)


     37 K.Nadarajah, Paul, D.C., J. Kader and W. M. Wan Yusoff (2005). The effects of soluble carbon sources on the production of chitosan by a new isolate of Rhizopus oligosporus. Malaysian Applied Biology 33(2):27-34


           38 Siti Aminah, S., Aidil, A. H. and Wan Mohtar, W. Y. (2006). Medium   

               Optimization for the production of lipidless biomass by Cunninghamalla sp.

               2A1 using response surface methodology. Malaysian Journal of Microbiology,  

               Vol 2(1), 40-45


     39 Nadarajah, K., Lau, B.Y.C., Othman, O., Hasidah, M.S. and Wan Mohtar,

         W.Y. (2006). Characterization of Chitin Deacetylase from fungus Absidia

         Butleri   dr. Malays. Appl. Biol. 35(2): 59-65


           40 Nur-Ainee Hayeeyusoh, Aidil Abdul Hamid and Wan Mohtar Wan    

               Yusoff (2007). A Simple Technique to Enhance the Productivity of delta-

               endotoxin in Batch  Culture. NU Science Journal; 4(1): 18-24



  • Contact Information :
    - Address
      Universiti Kebangsaan Malaysia
      43600 UKM Bangi
    - Phone Number
      6-03-89213808, 0193333921
    - Fax Number
    - E-mail